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Effect of Insulin Supplement on Development of Porcine Parthenogenetic Embryos KCI 등재

  • 언어ENG
  • URLhttps://db.koreascholar.com/Article/Detail/316151
  • DOIhttps://doi.org/10.12750/JET.2016.31.2.123
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한국동물생명공학회지 (구 한국수정란이식학회지) (Journal of Animal Reproduciton and Biotechnology)
한국동물생명공학회(구 한국수정란이식학회) (Journal of Animal Reproduction & Biotechnology)
초록

This study is performed to evaluate the effect of insulin in the porcine parthenogenetic embryo development. In porcine embryo culture, insulin is helpful factor in the process of embryo development. To identify this, insulin is used in pig embryos development. Therefore, this study was performed to investigate the effect of insulin on early embryonic development in pigs. For that, insulin positive or negative (0, 10 ug/mL) was supplemented in the porcine IVM media and then compared two groups divided by the cytoplasm of the black groups and white ring groups based on the distribution of lipid material of the cell cytoplasm in microscope. In maturation rates of porcine oocytes, significant higher black group rates were shown in the insulin positive groups compared with other groups (56.0±2.1 vs 46.2±0.3). In the embryo culture, black groups were showed the significant higher cleavage rates (82.1±0.8, 78.3±0.1 vs 63.2±0.3, 63.4±0.0), and blastocyst formation rates (15.5±3.6, 16.6±0.4 vs 11.7±1.3, 7.4±0.2) regardless of whether the addition of insulin. Also, black groups were showed higher cell number of blastocyst (33.2±2.5, 35.5±2.6 vs 31.2±2.1, 31.3±2.2). In conclusion, supplement of insulin producing black group in vitro maturation, it was effective in vitro maturation and embryonic development of pig embryos.

목차
INTRODUCTION
 MATERIALS and METHODS
  1. In vitro maturation of porcine oocytes
  2. Production of parthenogenetic embryos
  3. Experimental plans
  4. Statistical analysis
 RESULTS
  1. Effects of insulin on embryonic development in vitro maturationof porcine oocytes
  2. Developments of porcine parthenogenetic embryos
  3. Blastocysts cell number of porcine parthenogenetic pig oocytes
 DISCUSSION
저자
  • Youngkwang Yu(College of Veterinary Medicine, Chungnam National University)
  • Pantu Kumar Roy(College of Veterinary Medicine, Chungnam National University, Climate Change Disease Control Center, BrainKorea21 Plus)
  • Kyuhong Min(College of Veterinary Medicine, Chungnam National University)
  • Seungwon Na(College of Veterinary Medicine, Chungnam National University)
  • Euncheol Lee(College of Veterinary Medicine, Chungnam National University)
  • Ghangyong Kim(College of Veterinary Medicine, Chungnam National University, Climate Change Disease Control Center, BrainKorea21 Plus)
  • Xun Fang(College of Veterinary Medicine, Chungnam National University, Climate Change Disease Control Center, BrainKorea21 Plus)
  • MB Salih(College of Veterinary Medicine, Chungnam National University)
  • Jongki Cho(College of Veterinary Medicine, Chungnam National University, Climate Change Disease Control Center, BrainKorea21 Plus) Corresponding author