Dental pulp is a highly vascularized tissue with high regenerative potential. Revascularization of severed vasculature in the tooth is required for pulp healing during avulsed tooth treatment. In this study, the relative expression of angiogenesis-related proteins was determined in human dental pulp cells using a human angiogenesis proteome profiler array. The proteome profiler array detected differentially expressed angiogenesis-related factors under conditions of hypoxia, which enhances the angiogenic potential of dental pulp cells. We confirmed that hypoxia regulates the mRNA expression of angiogenesis-related factors, including CXCL16 in dental pulp cells. Furthermore, conditioned media of hypoxic pulp cells induced tube-like structures of vascular endothelial cells, which were reduced by the neutralization of CXCL16 function. In conclusion, our data show that angiogenesisrelated factors are differentially expressed by hypoxia in dental pulp cells and suggest that CXCL16 may involve in the revascularization of hypoxic dental pulp.

서 론
 실험재료 및 방법
  실험재료
  저산소 상태 (In vitro hypoxia)
  Proteome Profiler Human Angiogenesis Array
  RNA 분리와 중합효소연쇄반응 (PCR)
  관형성 실험 (Tube Formation Assay)
  RNA interference
 결 과
  치수세포에서 혈관신생 관련 단백질의 발현 분석
  정상 상태와 저산소 상태에서 치수세포 내 혈관신생관련 단백질의 발현 패턴
  저산소 상태에서 증가되는 혈관신생 관련 단백질이 혈관내피세포의 관형성에 미치는 영향
 고 찰
 References

• Mi-Kyoung Kim(Department of Oral Physiology, School of Dentistry, Pusan National University, Yangsan 626-870, South Korea)
• So-Jeong Kim(Department of Oral Physiology, School of Dentistry, Pusan National University, Yangsan 626-870, South Korea)
• Yeon Kim(Department of Oral Physiology, School of Dentistry, Pusan National University, Yangsan 626-870, South Korea)
• Hyun-Joo Park(Department of Oral Physiology, Department of Dental Pharmacology, School of Dentistry, Pusan National University, Yangsan 626-870, South Korea)
• Min-Jee Jo(Department of Oral Physiology, School of Dentistry, Pusan National University, Yangsan 626-870, South Korea)
• Soo-Kyung Bae(Department of Dental Pharmacology, School of Dentistry, Pusan National University, Yangsan 626-870, South Korea)
• Hyung Joon Kim(Department of Oral Physiology, School of Dentistry, Pusan National University, Yangsan 626-870, South Korea)
• Moon-Kyoung Bae(Department of Oral Physiology, School of Dentistry, Pusan National University, Yangsan 626-870, South Korea) Corresponding Author