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백진주의 저아밀로스 유전분석 및 분자지도 작성 KCI 등재

Genetic Analysis and Molecular Mapping of Low Amylose Gene in Rice Cultivar ‘Baegjinju’ (Oryza sativa L.)

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한국육종학회지 (Korea Journal of Breeding Science)
한국육종학회 (The Korean Breeding Society)
초록

The amylose contents of rice determine eating quality which is one of the major traits in rice breeding program. To identify the low-amylose gene of the japonica rice cultivar Baegjinju, genetic analysis was conducted using 200 F2 population derived from a cross between the japonica cultivars, Saeilmi and Baegjinju. Individual F2 plants were classified as wild type (translucent grain) and mutant type (dull grain) based on the grain appearance of brown rice. Two hundred F2 plants were segregated into 155 wild type plants and 45 mutant type plants, which fit the 3:1 ratio (x2 = 0.667, df = 1, p = 0.414) and this result indicated the low-amylose gene of Baegjinju is a single recessive gene which controls the amylose contents. Linkage analysis was conducted to localize the low-amylose gene of Baegjinju and fine mapped within an 800-kb interval between 17.5 to 18.8Mb on short arm of chromosome 10. Co-segregated SSR marker, RM25648 was developed and it could be useful for marker-assisted selection and determination of the genetic resource related with amylose contents in rice breeding.

목차
서 언
 재료 및 방법
  유전분석용 재료 양성
  Genomic DNA 추출 및 PCR
  유전분석 및 유전자지도 작성
 결과 및 고찰
  저아밀로스 유전분석
  저아밀로스 연관분석
  유전자지도 작성 및 분자마커 선발
 적 요
 REFERENCES
저자
  • 이종희(농촌진흥청 연구운영과) | Jong-Hee Lee
  • 조준현(국립식량과학원 남부작물부 논이용작물과) | Jun-Hyeon Cho
  • 이지윤(국립식량과학원 남부작물부 논이용작물과) | Ji-Yoon Lee
  • 손영보(국립식량과학원 남부작물부 논이용작물과) | Young-Bo Sohn
  • 한상익(국립식량과학원 남부작물부 논이용작물과) | Sang-Ik Han
  • 신동진(국립식량과학원 남부작물부 논이용작물과) | Dongjin Shin
  • 송유천(국립식량과학원 남부작물부 논이용작물과) | You-Chun Song
  • 박동수(국립식량과학원 남부작물부 논이용작물과) | Dong-Soo Park
  • 여운상(국립식량과학원 남부작물부 논이용작물과) | Un-Sang Yeo
  • 오명규(국립식량과학원 남부작물부 논이용작물과) | Myeong- Gyu Oh
  • 허연재(국립식량과학원 남부작물부 논이용작물과) | Yeon-Jae Hur Corresponding author