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NGS 기반 밀양23호/기호벼 재조합자식 유전집단(MGRIL)을 이용한 CAPS 마커 개발 및 줄기 굵기 형질 관련 QTL 분석 KCI 등재
Development of NGS-based New CAPS Markers and QTL Analysis of Stem Diameter using Milyang23/Gihobyeo Recombinant Inbred Lines (MGRIL)
pp.217-233
The rice recombinant inbred lines derived from Milyang23 and Gihobyeo cross were used in genetic mapping and QTL analysis studies. In this study, we developed a new 101 CAPS markers based on the SNPs in the whole genome region between these varieties. As a result, the total genetic distance and average distances were 1,696.97 cM and 3.64 cM, respectively. In comparison to the distance of the previous genetic map constructed based on 365 DNA markers, the new genetic map was found to have a decreased distance. The map was applied for the detection of QTLs on all seven traits relevant to diameter of stem internode, length of culms, length of panicles and the number of panicles including the correlation analysis between each trait. The QTLs results were similar to the report in previous studies, whereas the distance between the markers was narrowed and accuracy increased with the addition of 101 CAPS markers. A total of 9 new QTLs were detected for stem internode traits. Among them, qI1D-6 had higher LOD of 5.1 and phenotype variation of 50.92%. In this experiment, a molecular map was constructed with CAPS markers using next generation sequencing showing high accuracy for markers and QTLs. In the future, developing more accurate QTL information on stem internode diameters with various agriculturally important traits will be possible for further rice breeding.
재료 및 방법
벼(Oryza sativa L.)의 유전집단 육성 및 DNA 추출
NGS를 이용한 대량 염기서열 분석
SNP 프라이머 제작
CAPS 마커 개발 및 유전형 분석
물리 및 유전지도 작성
표현형 조사 및 QTL 분석
결과 및 고찰
CAPS 마커 개발
물리 및 유전지도 작성
표현형 상관관계 분석
벼의 7가지 형질 관련 QTL 분석
적 요
REFERENCES
