Genetic variations of γ-/ω-gliadin and Spa (storage protein activator) in 40 Korean wheat cultivars were evaluated to provide genetic information for improving end-use quality in wheat breeding programs. Korean wheat cultivars were classified into 13 patterns at the Gli-1 locus based on the allelic variation using A-PAGE (acidic-polyacrylamide gel electrophoresis). Seven, five, and six alleles were identified at Gli-A1, Gli-B1, and Gli-D1 loci, respectively. Allele-specific PCR markers for γ-gliadin corresponded to specific allele at Gli-1 loci on A-PAGE, which Gli-A1f, Gli-A1h and Gli-A1l alleles corresponded to GliA1.2, Gli-B1h and Gli-B1f alleles corresponded to GliB1.2 and Gli-D1f, Gli-D1m and Gli-D1o alleles corresponded to GliD1.1. DNA markers for γ-45 and γ-42 also corresponded to the γ-gliadin patterns around 40kDa on A-PAGE, except in Sukang, Ol and Joongmo2003. However, allelic specific PCR markers for ω5-gliadin did not correspond to that of A-PAGE. Three alleles were identified at Spa-A1 locus, whereas there was no variation at Spa-B1 and Spa-D1 loci.

서 언
 재료 및 방법
  공시재료
  A-PAGE를 이용한 글리아딘 분석
  PCR을 이용한 글리아딘 및 Spa 유전자 분석
 결과 및 고찰
  국내 밀 품종의 글리아딘 분석
  국내 밀 품종의 Spa 유전자 분석
 적 요
 REFERENCES

• 김지은(전북대학교 농업생명과학대학 작물생명과학과) | Ji-Eun Kim
• 윤영미(전북대학교 농업생명과학대학 작물생명과학과, 국립식량과학원) | Young-Mi Yoon
• 조성우(전북대학교 농업생명과학대학 작물생명과학과) | Seong Woo Cho
• 강천식(국립식량과학원) | Chon-Sik Kang
• 이종열(국립농업과학원) | Jong-Yeol Lee
• 박철수(전북대학교 농업생명과학대학 작물생명과학과) | Chul Soo Park Corresponding author