Rotaviruses are enteric pathogens causing acute watery dehydrating diarrhea in humans and animals. The importance of group C rotavirus (GpC-RV) infections has not been established as the studies on the GpC-RV have been hampered by the lack of an in vitro culture system. However, diarrheal diseases associated with GpC-RV have been gradually increasing worldwide. In this study, VP6 gene of bovine GpC-RV Korean isolate was expressed, and monoclonal antibodies (mAbs) against VP6 were produced and characterized. The VP6 gene was cloned and expressed based on a baculovirus expression system. Indirect fluorescence antibody (IFA), polymer chain reaction (PCR), and Western blot assays were used to confirm expression of VP6 gene synthesized by the recombinant baculovirus. Eleven mAbs against VP6 were produced using expressed VP6. Cross-reactivity of the mAbs was assessed with recombinant VP6 proteins from porcine GpC-RV and human GpA-RV, or different serotypes of group A rotavirus strains by IFA test. Some mAbs reacted with intact porcine GpC-RV Cowden strain as well as bovine GpC-RV VP6 recombinant baculoviruses, but not with human and animal GpA-RV strains. The VP6-specific mAbs might be useful to develop immunodiagnostic tests such as rapid diagnostic kit, IFA and enzyme-linked immunosorbent assay (ELISA) for detection of GpC-RV.

Introduction
 Materials and Methods
  Cloning and expression of bovine GpC-RV VP6 gene
  Monoclonal antibody against bovine GpC-RV VP6
 Results
  Cloning and expression of bovine GpC-RV VP6 gene
  Production and characterization of GpC-RV VP6 specific MAbs
 Discussion
 References

• Dong-Uk Kim(College of Veterinary Medicine, Chungbuk National University, Cheongju 26844, Korea, Chungcheongnam-Do Health and Environment Research Institute, Hongseong 32254, Korea)
• Seung-A Jeong(College of Veterinary Medicine, Chungbuk National University, Cheongju 26844, Korea)
• Seung-Chul Lee(Choongang Vaccine Laboratories, Daejeon 34055, Korea)
• Kyoung-Oh Cho(College of Veterinary Medicine, Chonnam National University, Gwangju, 61186, Korea)
• Shien-Young Kang(College of Veterinary Medicine, Chungbuk National University, Cheongju 26844, Korea) Corresponding author