Acorus gramineus is a herb used frequently in oriental medicine. Clinically, the effects of Acorus gramineus are known as awakening of one’s consciousness, removal of sputum, and relief of autism. Experimentally, Acorus gramineus root(AGR)-induced cerebral blood flow increase depending on the concentration causes diverse responses of blood pressure and regional cerebral blood flow and possesses strong inhibitory effects of apoptosis in the nervous system. We investigated the effects of AGR water extract on the adipose tissue status. Male C57BL/6 mice were fed high fat diet and a total of 35 mice were divided into 5 groups: normal group, control group, and groups treated with AGR water extract at concentrations of 20, 100, and 500 mg/kg. The mice were treated by oral administration once a day for consecutive 6 weeks, and their weight changes were monitored. As the results, the groups treated with AGR water extract showed weight loss. Also, the weights of liver, epididymal fat, retroperitoneal fat and peri-renal fat were decreased in the groups treated with AGR water extract. In histopathological examination, the sizes of adipose tissues decreased in liver, epididymal fat, retroperitoneal fat, and peri-renal fat. These results indicate that AGR water extract have an anti-obesity activity, which could be used as an useful material to decrease body adipose tissues.

Introduction
 Materials and Methods
  재료 및 추출
  실험동물
  군 구성
  간 및 체내 지방 상대 장기 중량 측정
  병리조직학적 검사
  통계학적 방법
 Results
  간의 상대 장기 중량 및 NASH 변화
  체내 지방 중량의 변화
  간의 병리학적 검사
  체내 지방조직의 병리학적 검사
 Discussion
 References

• Sunhwa Hong(Center for Animal Resources Development, Wonkwang University, Iksan, 54538, Korea)
• YeonShik Choi(Dept. of Bio-Medical Analysis Campus, Bio of Korea Polytechnics)
• Da-Seul Kim(Graduate School of Applied Animal Science, Wonkwang University, Iksan 54538, Korea)
• Hye-Young Kwon(Graduate School of Applied Animal Science, Wonkwang University, Iksan 54538, Korea)
• Okjin Kim(Center for Animal Resources Development, Wonkwang University, Iksan, 54538, Korea) Corresponding author