We developed a new ecotoxicological test method using native test species, eggs of Ephemera orientalis, and five kinds of industrial wastewater were tested to validate the test method. The water samples were collected in Jun 2006 from the following industries: pesticide, metal plating, PCB, leather1, and leather2. Wastewater and effluent were diluted by distilled water, respectively, to prepare various concentrations, 100, 50, 25, 12.5, 6.3, 3.1, and 0%. For the egg bioassay, 20 freshly laid eggs (<24 h old) were exposed to test solutions in a Petri dish (52×12 mm) at 20℃ with photoperiod of 16 h light and 8 h dark for 14 days. The median egg hatching concentrations (EHC50) were estimated using Probit analysis. All EHC50s of wastewater were less than 3.1%, which meant very high ecotoxicity except for the wastewater of PCB industry having 6.1% of EHC50. Among the effluents, the least toxic effluent was from pesticide industry having 58% of EHC50, while the effluent of leather2 was the most toxic having 7.3% of EHC50.

서 론
 재료 및 방 법
  1. 산업폐수 시료 채취 및 화학적 분석
  2. 동양하루살이 (Ephemera orientalis)를 이용한산업폐수 생태독성평가
 결과 및 고 찰
  1. 산업폐수 화학적 분석
  2. 동양하루살이 (Ephemera orientalis) 알을 이용한생태독성평가
 적 요
 REFERENCES

• 모형호(고려대학교 생명자원연구소) | Hyoung-ho Mo
• 손진오(고려대학교 환경생태공학부) | Jino Son
• 정진호(고려대학교 환경생태공학부) | Jinho Jung
• 신기일(한국외국어대학교 통계학과) | Key-Il Shin
• 조기종(고려대학교 환경생태공학부) | Kijong Cho Corresponding author