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Phospholipase C zeta 유전자의 유전적다형성과 돼지 액상정액의 운동학적 특성과의 연관성 분석 KCI 등재

Association study analysis of phospholipase C zeta gene polymorphism forsperm motility and kinematic characteristics in liquid semen of Boar

  • 언어KOR
  • URLhttps://db.koreascholar.com/Article/Detail/317352
  • DOIhttps://doi.org/10.12750/JET.2016.31.3.293
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한국동물생명공학회지 (구 한국수정란이식학회지) (Journal of Animal Reproduciton and Biotechnology)
한국동물생명공학회(구 한국수정란이식학회) (Journal of Animal Reproduction & Biotechnology)
초록

For evaluating the boar semen quality, sperm motility is an important parameter because the movement of sperm indicates active metabolism, membrane integrity and fertilizing capacity. Phospholipase C zeta (PLCz) is important enzyme in spermatogenesis, but the effect has not been confirmed in pigs yet. Therefore, this study was aimed to analyze their association with sperm motility and kinematic characteristics. DNA samples from 124 Duroc pigs with records of sperm motility and kinematic characteristics [total motile spermatozoa (MOT), curvilinear velocity (VCL), straight-line velocity (VSL), the ratio between VSL and VCL (LIN), amplitude of lateral head displacement (ALH)] were subjected. A SNP in non-coding region of PLCz g.158 A > C was associated with MOT (p < 0.05), VCL (p < 0.01), LIN (p < 0.01) and ALH (p < 0.05) in Duroc population. Therefore, we suggest that the intron region of the porcine PLCz gene may be used as a molecular marker for Duroc boar semen quality, although its functional effect was not defined yet. Whether the association is due to the candidate gene or not require further verification. Thus, it will be of interest to continue association studies in the regions surrounding those genes.

목차
서 론
 재료 및 방법
  정액 채취
  정자 운동학적 특성 분석
  Direct sequencing 및 유전자형 분석
  통계 분석
 결과 및 고찰
 REFERENCES
저자
  • 정용대(농촌진흥청 국립축산과학원) | Yong-dae Jeong
  • 정진영(농촌진흥청 국립축산과학원) | Jin-Young Jeong
  • 사수진(농촌진흥청 국립축산과학원) | Soo-Jin Sa
  • 김기현(농촌진흥청 국립축산과학원) | Ki-Hyun Kim
  • 조은석(농촌진흥청 국립축산과학원) | Eun-Seok Cho
  • 유동조(농촌진흥청 국립축산과학원) | Dong-Jo Yu
  • 박성권(세종대학교 생명과학대학 식품생명공학과) | Sungk-won Park
  • 장현준(단국대학교 약학대학) | Hyun-Jun Jang
  • 우제석(농촌진흥청 국립축산과학원) | Jae-Seok Woo Correspondence
  • 최정우(강원대학교 동물생명과학대학) | Jung-Woo Choi Correspondence