논문 상세보기
돼지 등심 내 카프르산 함량과 단일염기다형 마커 rs81437607 유전자형의 상관 KCI 등재
Association of the Genotypes of Single Nucleotide Polymorphism Marker rs81437607 with Capric Acid Contents in longissimus dorsi Muscle in Pigs
pp.235-242
This study tested the association between genotypes of the single nucleotide polymorphism (SNP) marker, rs81437607 and capric acid (FA_C10_0) compositions in longissimus dorsi muscle in pigs. Eighteen fatty acid (FA) compositions were measured in a total of 974 F2 animals among 1,106 F2 progeny produced between Landrace and Jeju Black Pig (JBP). Among FA compositions tested, we identified a cluster of highly significant SNPs for capric acid compositions on 58 Mb position of Sus scrofa chromosome 12 (SSC12) using genome-wide association study (GWAS) with F2 genotypes from SNP panel analysis. GWAS results showed that the rs81437607 was the highest trait-related SNP marker with capric acid levels. Three genotypes (C/C, C/T and T/T) of rs81437607 marker were found in F2 population by further pyrosequencing. Association analysis results showed the significant differences between rs81437607 genotypes and capric acid compositions (P<0.05). The F2 pigs harboring rs81437607 C/C (0.119±0.002%) and C/T (0.116±0.002%) genotypes showed additively higher levels of capric acid content than those of T/T homozygotes (0.109±0.002%) (P=1.30×10-12). These results suggested that the genetic variations of rs81437607 may be helpful to find causative variants and assist as molecular genetic markers for improving the capric acid contents in longissimus dorsi muscle in pigs.
재료 및 방법
1. 공시동물 및 DNA 분리
2. 카프르산 형질 측정 및 혼합모형분석
3. SNP chip panel 유전자형 분석과 GWAS 분석
4. 중합효소연쇄반응(PCR)과 유전자형 결정
5. rs81437607 g.58105269C>T 유전자형과 카프르산 함량의상관 분석
결과 및 고찰
1. 카프르산 함량에 대한 최고 연관 유전자 좌위와 SNP 마커검출
2. SNP 마커 rs81437607 g.58105269C>T의 유전자형의 빈도
3. 돼지 카프르산 함량에 대한 유전자 마커 rs81437607의 상관관계
4. 카프르산과 돼지의 질병 저항성
결 론
REFERENCES
