논문 상세보기
Cysteamine 첨가가 희소한우 OPU 및 도축난소 유래 난자의 발생에 미치는 영향 KCI 등재
The effects of cysteamine on in vitro production of embryos from rare breed hanwoo (albino White and Black) ovum pick-up and slaughterhouse derived oocytes
pp.191-197
Historically, Korea old cattle had been consisted with various lines of coat color brindle, black and white-brown breeds or more. The two rare lines of black and white coat color are maintained for animal resources and preserved critically. The present study was carried out to evaluate potential usage of cysteamine supplementation during in vitro matration (IVM) and in vitro culture/production of embryo (IVP) by transvaginal ultrasound-guided follicle aspiration (Ovum Pick-Up: OPU) for the establishment of cryo-banking system. Immature slaughterhouse-derived cumulus-oocyte complexes (SL-COCs) were matured in IVM medium supplemented with 0, 0.1, 0.3 or 0.9 mM cysteamine, and then cultured in mSOF-BAS for 8 days after in vitro fertilization. The treatment of 0.1 mM cysteamine on SL-COCs showed higher rate of blastocyst, so OPU-derived COCs from rare breeds were matured in TCM media supplemented with or without 0.1 mM cysteamine, FSH and 5% FBS. The embryos were evaluated their developmental stages on day 8. During IVM, cysteamine treatment significantly increased the embryo production rate of slaughterhouse-derived COCs (19.6% vs. 30.5%). The presence of cysteamine during IVM of OPU-derived COCs from rare Korean cattle breeds (albino white and black line) also increased embryo production rates than those from SL-COCs (27.4% vs. 41.9% and 36.4%). With these results, cysteamine treatment during IVM is one of key factors IVP of blastocysts to establish banking system of endangered rare Koarean cattle with OPU derived transferable blastocysts.
재료 및 방법
1. 시험축 선정
2. 난자 채취와 체외 성숙
3. 체외 수정과 발생
4. 자료 분석
결 과
1. cysteamine이 도축장 유래 난소의 체외 발생에 미치는 영향
2. OPU유래 난자 발생에 cysteamine이 미치는 영향
고 찰
결 론
REFERENCES
