During the ovary preservation in low temperature, the cumulus oocyte complexes(COCs) lose their developmental competences after in vitro fertilization. We used phosphate-buffered saline (PBS) as a basic solutions of at various temperatures (25, 15 or 5 ℃) and supplemented them with 1mM glucose and 0.5mM glutamine as a source of carbohydrate metabolites. After recovery of COCs and in vitro fertilization, a significantly higher number of oocytes developed into blastocysts. The developmental competence of embryos that were originated from ovaries preserved at 15 ℃ was increased compared to those of 25 or 5 ℃. The maturation rate of oocytes was not differed between 24 and 36 h at 15 ℃ but showed lower than control group (71% versus 78%). In vitro-fertilized oocytes from ovaries stored at 25 ℃ for 24 h or at 5 ℃ for 24 h had a significantly decreased developmental potentials, but at 15 ℃ did not (27% versus 29% of blastocysts to develop into day 8). With these results, bovine ovaries can be preserved at 15 ℃ for 36 h without decreasing developmental capacity of in vitro-fertilized oocyte at least to the blastocyst stage. This information provides valuable information of preserving ovaries for embryo transfer or in vitro embryo production.

서 론
 재료 및 방법
  1. 난소의 보존
  2. 난자의 회수율 및 성숙율 조사
  3. 체외 수정
  4. 체외 배양
  5. 자료 분석
 결 과
  1. 난소 저장 온도가 난자의 성숙에 미치는 영향
  2. 난소 저온 저장 시간이 난자의 성숙에 미치는 영향
  3. 난소 저장 온도가 난자의 발생에 미치는 영향
  4. 15℃에서 보존시간이 발생에 미치는 영향
 고 찰
 결 론
 REFERENCES

• 김성우(농촌진흥청 국립축산과학원 가축유전자원센터) | Sung Woo Kim Correspondence
• 김민수(농촌진흥청 국립축산과학원 가축유전자원센터) | Min Su Kim
• 김찬란(농촌진흥청 국립축산과학원 가축유전자원센터) | Chan-Lan Kim
• 김동교(농촌진흥청 국립축산과학원 가축유전자원센터) | Dongkyo Kim
• 김남태(농촌진흥청 국립축산과학원 가축유전자원센터) | Namtae Kim
• 성환후(농촌진흥청 국립축산과학원 가축유전자원센터) | Hwan-Hoo Seong