Recombinant baculovirus genome that absence part of ORF1629 has used to enhance efficiency of recombinant virus selection because a role of ORF1629 is believed to essential for viral replication in insect cells. It can be recovered by recombination with a transfer vector containing a complete ORF1629. Some recombinant bacmids were generated for this purpose. Recombinant bacmid GOZA, one of them, has a mini-F replicon for Escherichia coli and the partial ORF1629 gene. By accident, we could observe the replication of ApGOZA singly in Sf9 cells. Produced virus from ApGOZA was investigated for the existence of truncated ORF1629 not intact ORF1629 by PCR amplification and genomic sequencing. Also, we could observe the replication of AcGOZA alone in Sf9 cells. To analyze the influence of truncated ORF1629, the viral growth, BV production and polyhedral formation were conducted comparing to wild type virus and recombinat virus containing intact ORF1629. The results suggested the probability that ORF1629 is not essential for replication.

• See Nae Lee(Department of Agricultural Biology, College of Agriculture, Life & Environment Science, Chungbuk National University)
• Won Seok Gwak(Department of Agricultural Biology, College of Agriculture, Life & Environment Science, Chungbuk National University)
• Ji Hoon Lee(Department of Agricultural Biology, College of Agriculture, Life & Environment Science, Chungbuk National University)
• Soo Dong Woo(Department of Agricultural Biology, College of Agriculture, Life & Environment Science, Chungbuk National University)