The purpose of this study was to isolate an active constituent from the essential oil of Eugenia caryophyllata leaves and evaluate its insecticidal activity against Pochazia shantungensis nymphs and adults. According to chromatographic and spectroscopic analyses, the active constituent of E. caryophyllata leaves was identified as eugenol. Based on LC50 values and structural analogues of eugenola gainst the P. shantungensis nymphs and adults, isoeugenol (LC50, 83.29 and 91.03 mg/L) exhibited the highest insecticidal activity, followed by methyl isoeugenol (105.61 and 114.48 mg/L), eugenol (124.44 and 143.24 mg/L), methyl eugenol (126.31 and 143.84 mg/L), and acetyl eugenol (165.11 and 170.06 mg/L). Insecticidal activity against P. shantungensis was dependent on the presence of a functional group in 4-ally-2-methoxyphenol. These results indicate that E. caryophyllata oil and eugenol analogues might be potential alternative synthetic insecticidal agents.

• Ye-Jin Jeon(Department of Bioenvironmental Chemistry, Chonbuk National University)
• Jun-Hwan Park(Department of Bioenvironmental Chemistry, Chonbuk National University)
• Sang-Min Lee(Department of Animal Biotechnology, Chonbuk National University)
• Hui-Ju Kim(Department of Bioenvironmental Chemistry, Chonbuk National University)
• Hoi-Seon Lee(Department of Bioenvironmental Chemistry, Chonbuk National University)