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Exploration of Differential Gene Expression of Small Brown Planthopper, Laodelphax striatellus upon Buprofezin Treatments

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  • URLhttps://db.koreascholar.com/Article/Detail/317821
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한국응용곤충학회 (Korean Society Of Applied Entomology)
초록

The small brown planthopper (SBPH), Laodelphax striatellus, is one of the most serious pest insects of rice plants because it can transmit the rice stripe virus (RSV) which often causes significant reduction of yield in the field. Buprofezine is an effective insect growth regular (IGR) pesticide to control planthoppers, however, since the use of buprofezine for more than a decade, it has caused a certain resistance of SBPH. To survey the responses of SBPH to buprofezine, we exposed 4th instar SBPH to 200 ppm buprofezine by dipping method, and extracted total RNA for RNA-seq by Illumina platform. The quality filtered raw reads of cDNA obtained from experimental and control SBPH were subjected to Bowtie2 followed by eXpress computer program to compare the differential gene expression which will be important information for pest control methods using RNAi.

저자
  • Ying Fang(Department of Agricultural Biotechnology, Seoul National University)
  • Seok-Hee Lee(Department of Agricultural Biotechnology, Seoul National University)
  • Jong Hoon Kim(Department of Agricultural Biotechnology, Seoul National University)
  • Dong Hwan Park(Department of Agricultural Biotechnology, Seoul National University)
  • Min Gu Park(Department of Agricultural Biotechnology, Seoul National University)
  • Ra Mi Woo(Department of Agricultural Biotechnology, Seoul National University)
  • Woo Jin Kim(Department of Agricultural Biotechnology, Seoul National University)
  • Jae Young Choi(Research Institute for Agriculture and Life Sciences, Seoul National University)
  • Yeon Ho Je(Department of Agricultural Biotechnology, Seoul National University, Research Institute for Agriculture and Life Sciences, Seoul National University)