Varroa destructor is a devastating ectoparasitic mite which attacks Honeybee, Apis mellifera. V. destructor feeds on honeybee hemolymph, and often harbors small RNA viruses such as the deformed wing virus to transmit these viruses in the infested bee hive. To survey the genes of V. destructor, total RNA was subjected to high-throughput transcriptome sequencing to construct in silico cDNA library by using the Illumina HiSeq 2000 platform. Total of 2×107,748,792 paired-end short reads were obtained and quality filtered reads were subjected to Trinity de novo assembler followed by TransDecoder, and CD-HIT program to make a V. destructor reference cDNA library containing 28,023 of clustered contigs with protein coding capacity. These cDNA sequences will help us to understand the molecular biology of V. destructor.

• Woo Jin Kim(Department of Agricultural Biotechnology, Seoul National University)
• Seok Hee Lee(Department of Agricultural Biotechnology, Seoul National University)
• Fang Ying(Department of Agricultural Biotechnology, Seoul National University)
• Jong Hoon Kim(Department of Agricultural Biotechnology, Seoul National University)
• Dong Hwan Park(Department of Agricultural Biotechnology, Seoul National University)
• Ra Mi Woo(Department of Agricultural Biotechnology, Seoul National University)
• Min Gu Park(Department of Agricultural Biotechnology, Seoul National University)
• Jae Young Choi(Research Institute for Agriculture and life Sciences, Seoul National University)
• Yeon Ho Je(Department of Agricultural Biotechnology, Seoul National University)