Potato Virus Y (PVY) (Potyviridae: potyvirus) is one of the serious emerging virus of seed potato world-wide. It affects the seed potato by transmitting non-persistently via aphids. Here, we developed a simple PVY detection method which used the boiling technique for releasing of the viral RNA from aphid such as stylet and amplification by PVY specific primers located in the viral coat protein gene which suitable for various strains. This simplified method could save the time compared to earlier detection method due to the simplified RNA extraction step. Following this procedure, we tested this one step RT-PCR based PVY detection method by using three PVY vectoring aphid species (M. persicae, A. gossypii and M. euphorbiae) as well as other sucking type insect such as thrips (F. occidentalis). This PVY detection method is rapid, easy-to-use and suitable for large-scale testing in laboratories of seed potato.

• Juil Kim(Highland Agriculture Research Institute, NICS, RDA, 25342 Pyeongchang, Rep. of Korea, Department of Entomology, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany)
• Deok Jea Cha(Highland Agriculture Research Institute, NICS, RDA, 25342 Pyeongchang, Rep. of Korea)
• Min Kwon(Highland Agriculture Research Institute, NICS, RDA, 25342 Pyeongchang, Rep. of Korea)