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Co-culture with Good Quality-COCs Enhances the Maturation and Development Rates of Poor Quality-COCs
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/318139
In pig, more than half of the recovered cumulus cell-oocyte complexes (COCs) have one or two layers of cumulus cells and are considered morphologically poor. If we could take full advantage of these poor quality COCs, we could potentially improve the efficiency of in vitro embryo production. During in vitro maturation, although some maturation factors are transmitted bidirectionally between the oocyte and cumulus cells of the same COC, transmission also occurs between different COCs. We hypothesized that morphologically poor COCs fail to undergo complete oocyte maturation due to their insufficient secretion of maturation factors. Here, we investigated whether co-culture with morphologically good COCs (having three or more layers of cumulus cells) could improve the maturation and utilization rates of morphologically poor COCs. Our results revealed that the oocyte maturation rate, glutathione level, embryo development capacity, blastocyst quality, and cumulus cell gene expression levels of BCL-2 and PCNA were similar in the co-culture and good quality-groups, and that these levels were all significantly higher than those in the poor quality-group. Our results strongly suggest that the co-culture strategy greatly improved the utilization rate of morphologically poor COCs without reducing their capacity for maturation and subsequent development.
