The uniform nanofibers of polyurethane with different contents of Juniperus Chinensis extracts were successfully prepared by electrospinning method. Polyurethane is widely used as functional polymers in the industrials, medical field as their properties can be tailor-made by adjusting their compositions. Juniperus Chinensis has been reported for anti-tumor, anti-bacterial, anti-fungal, and anti-viral activities. PU/Juniperus Chinensis extracts composite nanofibers were produced at different Juniperus Chinensis extracts concentrations (0.25, 0.5, 1, 1.5wt.%). The effects of the major parameters in electrospinning process such as tip to collector distance (TCD), voltage, polymer concentration on the average diameter of electrospun nanoweb were investigated. As results, 12wt% PU solution concentration, 8kV applied voltage and 15cm tip to collector distance were identified as optimum conditions for electrospinning the composite nanofibers. The diameter and morphology of the nanocomposite nanofibers were confirmed by a scanning electron microscopy (SEM). The resulting fibers exhibited a uniform diameter ranging from 435nm∼547nm. It has been found that the average diameters of fibers decreased by the adding of Juniperus Chinensis extracts. These nanowebs can be used for medical materials, protective clothing, and antimicrobial filters.