Objectives
The objective of our research was to establish the gene transformation, expression and characterization system in transgenic Codonopsis lanceolata.
Materials and methods
Agrobacterium tumefaciens strain LBA 4404/ binary vector pYBI121Regeneration of transgenic shoots: MS medium supplemented with 0.1 mg/ℓ NAA and 1 mg/ℓ BAP, 3% sucrose and 0.8% agar at pH 5.8. Agrobacterium cell density OD 600 between 0.8 and 1.0, Infection: 5 minutes DNA isolation and Polymerase chain reaction: DNA was extracted from young leafs excised from kanamycin resistant shoots. Two primers used for PCR amplification of the 700 bp of the npt II gene were N 1 (5′ GAA GCT ATT CGG CGG CTA TGA CTG 3′) as a sense primer and N 2 (5′ ATC GGG AGC GGC GGC GAT ACC CTA 3′) as a anti sense primer.
Result and Discussion
Adventitious shoots regenerated 3 weeks after Agrobacterium infection on regeneration medium
containing 0.1 mg/ℓ NAA and 1 mg/ℓ BAP, 100 mg/ℓ kanamycin 250 mg/ℓ cefatoxime.
Numerous adventitious shoot inductions of putative transformants were observed from the cut
surface of explants which initially resembled knob like structure and later developed into new
plant. PCR analysis of showed the expected bands of npt II gene. PCR analysis was carried out
to confirm the insertion of the npt II gene in the genome of transformed plant. The expected
amplified npt II fragments of size 700 bp was found in the T0 transformed plants, indicating the
integration of npt II gene.

• Bimal Kumar Ghimire(Division of Bio‐Resource Technology, Kangwon National University) Corresponding author
• Eun Won Seo(Division of Bio‐Resource Technology, Kangwon National University)
• Jung Dae Lim(Division of Bio‐Resource Technology, Kangwon National University)
• Chang Yeon Yu(Division of Bio‐Resource Technology, Kangwon National University)