Heading date in rice is a complex trait that is governed by multiple genes and environmental factors, such as day-length, temperature, and soil conditions. The genetic studies using DNA markers have facilitated the genetic dissection of heading date and many quantitative trait loci (QTLs) for heading date have been identified using several mapping population. In a previous study, a new quantitative trait loci (QTLs) for heading date have been identified using several mapping population. In a previous study, a new for heading date was detected near SSR marker RM215 on chromosome 9 using an advanced backcross line, WH29001, developed by introgressing chromosomal segments from an accession of Oryzaminuta (2n=48, BBCC, Acc. No.101141)into the O. sativa subsp. japonica cv. Hwaseongbyeo. The O. minuta allele of QTL contributed to an increase in heading date. To clarify whether dth9 could be dissected genetically, a high-resolution linkage mapping of dth9 was performed using alarge F2 population derived form the cross between one F4 plant which was homozygous for O.minuta in the target region RM5661-RM215 on chromosome9 and Hwaseongbyeo. Days to heading in the F2 population showed continuous variation rang form 102 to 113 days. The dth9 QTL further narrowed down at the interval between the SSR marker RM1553 and RM215 which was approximately 403kb in length based on the physical map of the region. The QTL for heading date(dth9) had not been detected in previous QTL studies between Oryza cultivars, indicating the existence of potentially novel alleles from O. minuta.