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Genetic mapping of anthocyanin biosynthetic genes in soybean seed coat. KCI 등재
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/318285
Proanthocyanidins and anthocyanins derived from the phenylpropanoid pathway most likely play a protective function from pathogens and UV light exposure within the plant and, in addition, act as signal molecules in plant-microbe interactions. The metabolites are now attracting attention because of the medicinal and nutritional values due to their antioxidant properties and flavors. Three independent loci (I, R, and T) control pigmentation of the seed coats determined by proanthocyanidins and anthocyanins in soybean (Glycine max). The I locus controls distribution of anthocyanin and proanthocyanidin pigments, which in its dominant form exhibits homology-dependent gene silencing leading to a yellowish seed coat. The R and T genes determine the anthocyanin and proanthocyanidin products and specific seed coat color such as black, imperfect black, brown, or buff. The I and T loci have been cloned. The objectives of this study were to develop PCR-based molecular markers cosegregating with the genetic loci controlling the biosynthesis of these interesting metabolites using public soybean EST and genomic sequence data and is to develop molecular markers to establish a marker-assisted selection scheme for these natural products-related traits. A population of 112 F11 recombinant inbred lines generated by an interspecific cross between a Glycine max line 'Hwangkeumkong' and a G. soja Siebold & Zucc. line ‘IT182932' was used to construct a frame map consisting of 20 soybean linkage groups. The frame map contains over 300 SSR, RAPD and transposon markers. PCR-based molecular markers cosegregating with the I and T loci that control pigmentation of the seed coats determined by secondary metabolites derived from the flavonoid pathway including anthocyanins and proanthocyanidins in soybean have been developed. We have developed three SSR cosegregating with the I locus and one codominant STS and one SNP markers cosegregating with the T locus. So far, we have developed SSR, SNP, and STS markers cosegregating with the I locus and with the T locus. Work is in progress to develop markers cosegregating with other genetic loci. The markers will facilitate markers-assisted selection of seed coat colors in molecular breeding programs.
