논문 상세보기
Isolation and Characterization of Various Genes from Panax ginseng C. A. Meyer KCI 등재
Ginseng (Panax ginseng C. A. Meyer), a perennial herb in the Araliaceae family, is one of the most commonly utilized medicinal plants in the world. Because whole genome sequence of the ginseng plant is not analyzed, the expressed sequence tags (ESTs) from a ginseng plant was studied by constructing cDNA libraries. Almost 20,000 ESTs were collected and BLAST comparisons of the cDNAs in GenBank’s non-redundant databases revealed that many cDNAs (89.1%) exhibited a high degree of sequence homology to genes from other organisms. The majority of the identified transcripts were found to be genes related with energy, metabolism, subcellular localization, and protein synthesis. Many cDNA clones containing fructose-1,6-bisphosphate aldolase, dehydrin, catalase, and glutathion-S-transferase etc. were analyzed to study their characteristics. The expressions of the genes in different organs were analyzed using reverse transcriptase (RT)-PCR. In addition, the expressions of the genes under different abiotic stresses were analyzed at different time points.
Ginseng Genetic Resource Bank (GGRB) in Kyung Hee University is the center constructed for ginseng genomic and genetic information. GGRB collects genetic resources of ginseng cultivars with systematic management system for effective conservation and application of the resources. GGRB supplies researchers new ginseng cultivars, specific markers, ginseng DNA and RNA for scientific researches.
