The object of this study was to use genomic information obtained from wheat-rice sequence to develop genome-specific PCR primer for Agp-L gene involved in starch biosynthesis. Intron locations in wheat were inferred through alignment of wheat cDNA sequence of Agp-L with rice genomic sequence. Exon-anchored primer which amplify across introns allowed sequencing of introns from the three genomes provided the basis for genome-specific primer design. The single nucleotide polymorphism (SNP) was identified and this SNP could be converted into cleaved amplified polymorphism sequence (CAPS).

• Hwa-Young Heo(Suwon, National Institute of Crop Science, RDA) coresponding author
• Nancy Blake(Bozeman, Montana State University)
• Hong-Sik Kim(Suwon, National Institute of Crop Science, RDA)
• Dea-Wook Kim(Suwon, National Institute of Crop Science, RDA)
• Kwang-Geun Park(Suwon, National Institute of Crop Science, RDA)
• Si-Ju Kim(Suwon, National Institute of Crop Science, RDA)