Anthocyanins of black soybean may play an important role in physiological functions related to human health such as antioxidant, anti-inflammatory, and anticancer. The objective of this study was to investigate the profiles of anthocyanin in fourteen black soybean varieties using high-performance liquid chromatography (HPLC) with photodiode array detector (PAD). In all of the cultivars analyzed totally nine anthocyanins including, CatCy3glc, Dp3gal, Dp3glc, Cy3gal, Cy3glc, Pt3glc, Pg3glc, Pn3glc, and Cy were found. Cy3glc was the major anthocyanin content, represention 69.5% of anthocyanin, followed by Dp3glc (23.0%), Pt3glc (4.9%), Pn3glc (1.2%), and Pg3glc(1.1%), respectively. In contrast, the other five anthocyanins were in very low amounts, below 0.3 % in all varieties. Comparing cultivars and anthocyanin compositions, Geomjeong 2, Cheongja 2, and Cheongja 3 were found to content 9 anthocyanins. Geomjeong 1 and Seonheuk contented CatCy3glc, Cy3gal, Cy3glc, and Pn3glc. The variation of total anthocyanin concentration were significant for soybean cultivars. Geomjeong 2 showed highest average total anthocyanin content (17,937.8 ㎍/g seed coat), and Tawonkong and Heugcheong had the lowest total anthocyanin values (2,835.7 and 2,853.1 ㎍/g seed coat, respectively).