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Molecular characterization of soybean DnaJ-like protein induced by heat shock KCI 등재

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  • URLhttps://db.koreascholar.com/Article/Detail/318673
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한국작물학회 (Korean Society Of Crop Science)
초록

We isolated wound-inducible genes using suppression subtractive hybridization (SSH) method and were able to obtain to clone W3 gene encoding dnaJ like protein. The full-length cDNA of W3 is 689 bp with an open reading frame (ORF) consisting of 163 amino acid (aa). Genomic southern blot confirmed that soybean genome has two copies of W3 gene. Northern blot analysis was also carried out for the gene expression during heat, NaCl, drought, wounding stresses. The expression of W3 gene specifically induced by heat, NaCl, wounding and drought stress. Using GFP fusion vector, W13-GFP was targeted both to nucleus. For the functional analysis of W3, His-tagged W3 recombinant protein was heterologously expressed in E. coli. The W3 recombinant cells showed enhanced heat tolerance compared to that of vector control cells. We suggest that dnaJ-like W3 protein function as molecular chaperone in the nucleus of the plant cell during various stresses.

저자
  • Hyun-A So(BK21 Center for Silver-Bio Industrialization, College of Natural Resources and Life Science, Dong-A University)
  • Eunsook Chung(BK21 Center for Silver-Bio Industrialization, College of Natural Resources and Life Science, Dong-A University)
  • Chang-Woo Cho(BK21 Center for Silver-Bio Industrialization, College of Natural Resources and Life Science, Dong-A University)
  • Jee-Sook Kang(BK21 Center for Silver-Bio Industrialization, College of Natural Resources and Life Science, Dong-A University)
  • Kyoungmi Kim(BK21 Center for Silver-Bio Industrialization, College of Natural Resources and Life Science, Dong-A University)
  • Jai-Heon Lee(BK21 Center for Silver-Bio Industrialization, College of Natural Resources and Life Science, Dong-A University) Corresponding Author