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Cloning and molecular analyses of mungbean (Vigna radiata L.) 2-Cys Prx KCI 등재
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/318682
We isolated low temperature inducible genes using suppression subtractive hybridization (SSH) method and were able to obtain to clone MLT7 gene encoding peroxiredoxin and aminotransferase. The full-length cDNA of MLT7 is 1,049 bp with an open reading frame (ORF) consisting of 261 amino acid (aa). Genomic southern blot confirmed that mungbean genome has two copies of MLT7 gene. Northern blot analysis was also carried out for the gene expression during ABA, NaCl, drought, wounding and H2O2 stresses. The expression of MLT7 gene significantly decreased by ABA, NaCl and drought stress, but wounding and H2O2 stress significantly induced MLT7 gene expression. Especially, H2O2 strongly induced the MLT7 gene expression. The expression of MLT7 gene during low temperature stress started to increase in 3 h after treatment, and than slightly decreased and again increased at 24 h. Using GFP fusion vector, GFP-MLT7 was targeted both to mitochondria and chloroplast. However, it was mostly targeted to mitochondria and partially targeted to chloroplast. For the functional analysis of MLT7, MLT7 recombinant protein was heterologously expressed in E. coli. The MLT7 recombinant cells showed enhanced antioxidant activity compared to that of vector control cells.
