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Evaluation of seed storage protein subunits for providing selection criteria in malting barley breeding program KCI 등재
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/318703
Seed storage proteins of different solubility were extracted and denatured subunits of each protein were evaluated with malting barley quality parameters. Its been known that each subunit of seed storage protein encoded by each gene and subunit profiles were highly related to end-use quality in cereals. The purpose of this study is to provide selection criteria for high quality malting barleys with aid of bichemical-genetic information. Total 13 regional test lines and three locations (Naju, Jinju, and Jeju) were incorporated in this study. Albumin and hordein were extracted, denatured, and separated in 12% SDS-PAGE. Presence and absence of subunits of each protein were scored. Dendrogram (using XLSTAT program) was constructed to evaluated the relatedness of lines. The correlation between band profiles and quality test were assessed through Agglomerative Hierarchical Clustering (AHC) for statistics analysis. Hordein subunits can be classified into four groups, A, B, C, and D group. In general, hordein fractions contribute higher than albumine to determine malting quality. Specific molecular weight ranges (97.4-31.0, 66.2-31.0, and 45.0-31.0 kDa) of subunits were highly correlated with malting barley quality parameters. The subunit information would be directly incorporated in providing selection criteria for high quality malting barley in the malting barley breeding program.
