Japonica rice cultivars exhibit high susceptibility to BB disease due to genetic vulnerability in Korea. Korean Japonica rice cultivars mainly posses the genes, Xa1 and Xa3 for BB resistance. These resistance genes are becoming susceptible to K3a, new races of BB, resulting in the breakdown of resistance in high yielding Japonica cultivars. It is imperative to look for novel R-genes for improvement of japonica rice resistant to BB races. This study was carried out to conform useful single gene resistant to 24 BB isolates (including K3a, HB01009) breaking down Xa3 gene. Cultivars and near-isogenic lines (NILs) carrying Xa1, Xa2, xa8, Xa10, Xa11, xa13 genes were susceptible to 24 isolates, whereas IRBB4 carrying Xa4 gene was moderate resistance. IRBB5 and IRBB21 having xa5 and Xa21 genes, respectively, expressed resistance to these isolates. IRBB7 having Xa7 gene showed resistance response to 24 BB isolates, whereas JBB-107 carrying Xa7 gene was susceptible to 10 BB isolates and moderate resistant to 14 BB isolates. Xa7 gene showed different resistance response according to genetic background of used recurrent parent. With these findings, Xa4, xa5, and Xa21 would be the most prospective genes to 24 isolates used in screening.