논문 상세보기
Ecotype classification of backcross recombinant inbred lines of a cross between Japonica and Tongil-type rice varieties KCI 등재
BC1F7 RILs were cultivated and harvested in field. Two methods were adopted to classify ecotype of the RILs. First method was based on the seed length, width, length/width ratio, phenol reaction of the seed, and response to potassium chlorate of young seedling. Second was the classification using the polymorphism in SSR analysis. The results of UPGMA cluster analysis indicated that 168 RILs were classified into two ecotypes, 32 Japonica and 136 Tongil-type lines when first method was adopted. When second method was adopted, 35 and 99 lines among 134 RILs tested were belonged to Japonica and Tongil-type, respectively. The RILs belonged to same ecotype in both methods was 65.6% in Japonica, 91.9% in Tongil-type and 83.6 % in overall. The parents and BC1F7 RILs showed polymorphism in 20 among 21 RM primers used in SSR analysis. The proportion showing band pattern of each primer corresponded with ecotypes grouped by two methods ranged 29.7 to 99.1%. RM131, RM124, RM567, RM559, and RM348, which are located on chromosome 4, showed high proportion of correspondence over 94%. It was suggested that they would be used as molecular markers for rice ecotype classification. Japonica RILs grouped by two methods showed shorter grain length, no seed response to phenol solution and higher resistance to potassium chlorate solution in seedling stage compared with those of Tongil-type lines.
