Medicinal soybean, which has been proven to be effective in preventing cancer, heart disease, osteoporosis, and renal failure, is drawing people’s attention among other beans in recent days. This experiment was to examine the concentration of phenolic compounds and isoflavone in 53 kinds of soybeans cultivated and collected in Yeo-Ju, Gyeonggi-do region. The total average content of phenol is 29424.34㎍/g and the most and least phenol were detected in No. 35(814.98㎍/g) and No. 28(353.66㎍/g) respectively. Myricetin was detected the most resveratrol whereas detected the least amount which was less than 0.2% of the total amount among all medicinal soybean samples. Total average isoflavone content was 35910.56㎍/g and the most and least isoflavone was detected in No. 15 (996.66 ㎍/g ) and No.9 (476.18㎍/g )respectively . Malonylgenistin was detecteded the most genistein whereas detected the least amount which was less than 0.02 % of the total amount in all medicinal soybean samples. The result of this experiment is considered to be the basic data of the functional food manufacturing by utilizing domestic medicinal soybean.