This study was carried out to identify the quantitative trait loci (QTLs) for traits related to cold tolerance using an introgression lines (ILs) derived from a cross between a japonica weedy rice and a Tongil-type rice. Among the 80 RILs, one line (CR1835) showing tolerance for cold tolerance related to traits such as panicle exsertion and discoloration in the cold water plot was selected and backcrossed to the recurrent parent, Milyang 23. This line possessed Hapcheonaengmi 3 segments on chromosomes 1, 5 and 11. By two backcrosses to Milyang 23 and selfing, a total of 88 BC3F5 progenies were developed. The 88 ILs were evaluated for traits related to agricultural performance in cold water and in control plots. Cold tolerance was measured as difference of the culm length, spikelet fertility between two plots and panicle exsertion in cold water plot. The 88 ILs showed decreased culm length and increased anthocyan content in cold water plot than in the control water plot.