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Isolation and Sequencing of Homologous TaVRT2 Genes in Wheat (Triticum aestivum) KCI 등재
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/319694
Flowering is one of the most important developmental programs that plants use to ensure survival and reproductive success. The timing of flowering is under the control of several interdependent pathways. The molecular and genetic background of the interaction between environmental factors and the floral transition in these cultivars are still not reported. TaVRT2 expression is up-regulated in the winter genotypes during the vegetative phase and in photoperiod-sensitive genotypes during short days, and is repressed by vernalization to a level that allows the transition to the reproductive phase. Protein-protein interaction studies revealed that TaVRT-2 interacts with proteins encoded by two important vernalization genes (TaVRT-1/VRN-1 and VRN-2) in wheat. These results support the hypothesis that TaVRT-2 is a putative repressor of the floral transition in wheat. This gene is located on the short arm of homologous group 7 chromosomes in hexapolid wheat. The TaVRT2 acts as a repressor of the floral transition in wheat. We found that the flowering suppressor of flowering suppressor gene might be located on the short arm of chromosome 7D using several chromosomal substitution or aneuploid lines. The genetic map has been constructed from segregation of 370 SSR loci using 210 recombinant inbred lines (RILs), which was established at F7 eneration by the single-seed descent method from F2 family derived from Mironnovavskaya 808 and Chinese Spring. To perform mapping on the TaVRT2 on the homologous group 7 chromosomes, three homogous genes, TaVRT-1, TaVRT-2, TaVRT-3, are isolated from two common wheat cultivars; Chinese Spring (CS), Mironnovavskaya 808 (M808) and nucleotide polymorphisms between two cultivars are detected.
