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Increase of Resveratrol Content and Gene Expression Level According to Days after Seedling in Transgenic Rice KCI 등재

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  • URLhttps://db.koreascholar.com/Article/Detail/319699
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한국작물학회 (Korean Society Of Crop Science)
초록

Resveratrol (3,5,4'-trihydroxystilbene) is a naturally occurring antioxidant found in grapes, grape products such as wine, and some other botanical sources, like peanuts. In grapes, resveratrol occurs both as free resveratrol and piceid, which is 3-β-mono-D-glucoside of resveratrol. In recent years, research on resveratrol has discovered several beneficial biological effects of this compound to human health. These include anticancer activity, cardioprotection, antioxidant activity, inhibition of platelet aggregation, and antiinflammatory activity. So, we conducted this study to develop transgenic rice plants an enhanced phytochemicals associated with reduced cancer risk by overexpression of a peanut RS gene and selected 2 lines, 'Iksan 515' and 'Iksan 526', according to agronomic traits of rice plant and resveratrol contents in rice grain. Resveratrol content of Iksan 515 was increased by 2.3 times until 5 days after seedling and decreased at 7 days after seedling. Expression level of RS gene according to seedling days by Real-time PCR increased until 5 days, but decreased by half at 7 days. Now, we are planning to analyze medicinal function of these high resveratrol rices and development of cultivation technology to increase resveratrol content. Therefore, transgenic rices producing high resveratrol can be used to develop new rices with a cancer chemopreventive role in human medicine.

저자
  • Woon-Chul Shin(Department of Rice and Winter Cereal Crop, NICS, RDA) Corresponding author
  • So-Hyeon Baek(National Institute of Crop Science, RDA)
  • Chun-Sun Seo(Department of Rice and Winter Cereal Crop, NICS, RDA)
  • Ki-Young Kim(Department of Rice and Winter Cereal Crop, NICS, RDA)
  • Jong-Cheol Ko(Department of Rice and Winter Cereal Crop, NICS, RDA)
  • Young-Jun Mo(Department of Rice and Winter Cereal Crop, NICS, RDA)
  • Jeong-Kwon Nam(Department of Rice and Winter Cereal Crop, NICS, RDA)
  • Bo-Kyeong Kim(Department of Rice and Winter Cereal Crop, NICS, RDA)
  • Jae-Kwon Ko(Department of Rice and Winter Cereal Crop, NICS, RDA)