To develop the STS (Sequence Tagged Site) marker for discrimination between oat cultivars used the EEG (Euchromatin Enriched Genomic) DNA library in oat. The EEG DNA library was constructed the Mcr A and Mcr BC system in DH5 alpha bacterial cell line. About 3,500 EEG colonies had been constructed by using junk DNA exclusion. About 800 colonies were selected that included insert DNA more than 500 bp. It was analyzed the genetic information by using blast searches of NCBI web site. More than three hundred STS primer sets were developed using sequencing data of selected colonies and about 90 primer sets which showed single band were selected in Olgwiri. It was applied to twelve oat cultivars including Olgwiri and has been shown polymorphism at 15%. PCR product which amplified with selected STS primer was treated with six endonucleases and was showed polymorphic bands. These primers could be useful for specific allele tagging in mapping populations and germplasm and for the study of functional genomics of oat.

• Jun-Cheol Moon(National Institute of Crop Science, RDA)
• Hyung-Ho Park(National Institute of Crop Science, RDA) Corresponding author
• Poo-Reum Oh(Monsanto Korea, Inc)
• Jong-Jin Hwang(National Institute of Crop Science, RDA)
• Dea-Wook Kim(National Institute of Crop Science, RDA)
• Jang Hoon Sung(National Institute of Crop Science, RDA)
• Kwang-Geun Park(National Institute of Crop Science, RDA)
• Ji-Ung Jeong(National Institute of Crop Science, RDA)
• Wook-Han Kim(National Institute of Crop Science, RDA)