We developed an efficient protocol for transformation of balloon flower (Platycodon grandiflorum) root cultures by using cotyledon leaf explants that were infected by Agrobacterium rhizogenes. We found that four different strains of A. rhizogenes differed in their ability to transform P. grandiflorum hairy root cultures. We also found correct antibiotics concentration for selection after transformation by Agrobacterium. Our results demonstrate that use of suitable strain of A. rhizogenes and correct level of antibiotics for the hairy root culture, and may allow to study and apply of valuable metabolites like phenolic compounds from P. grandiflorum hairy root culture.

• Pham Anh Tuan(Division of Plant Science and Resources, Chungnam National Universit)
• Nam Il Park(Division of Plant Science and Resources, Chungnam National University)
• Xiaohua Li(Division of Plant Science and Resources, Chungnam National University)
• Yong Kyoung Kim(Division of Plant Science and Resources, Chungnam National University)
• Sang Un Park(Division of Plant Science and Resources, Chungnam National University) Corresponding author