This experiment was conducted to develope optimum cultural system of sesame under greenhouse condition with the comparison of open field cultural condition. The relatively higher adaptable varieties under greenhouse condition were white sesame "Pyoungan" and black sesame "Jinki" which showed higher yield potential and disease resistance. To set up standardized cultural system under greenhouse condition, we conducted several experiments of optimum sowing date, planting density and pinching time etc. When it came to sow sesame seed soon after early cultivated crops were harvested, the yield potential was higher than those of other sowing dates. Therefore, "June 30th" was optimum sesame sowing date in the greenhouse cultivation system. We also found out the space of 40㎝ between rows and 30cm(a few branch type) or 40cm(many branch type) between plants was better than those of other treatments. Optimum pinching time was 25 days after first flowering date when 1000 seeds weight and yield potential showed higher than other treatments.