This experiment was conducted to develope optimum seedling quality for the sesame transplanting system. To determine optimum seedling quality condition, we conducted several experiments. At the optimum pot selection experiment for the sesame transplanting, plastic pot with 105 holes showed relatively better early growth status of sesame than those of other plastic pots with 72, 128, 164 and 200 holes. And the soil combination, 'Bunong + Plant World' which is mixed 50% respectively showed higher germination rate, lower disease and lodging rate than those of other nursery soils at the determination of optimum nursery soil mixtures. We also conducted one experiment to find out seedling duration for the optimum transplanting seedling quality. According to the result, the seedling stage with about 4 or 5 regular leaves which came to 40 days after sowing showed higher number of capsule per plant and yield potential. Those results will help to develop mechanized sesame seedling transplanting system to reduce labor and increase net income for the sesame cultivation farmers.