This study was conducted to evaluate the feeding value of black soldier fly pupa (BSFP) on fecal microorganism, immunoglobulinm egg quality and egg production laying hens. One hundred twenty laying hens were assigned to 4 treatments (0, 5, 7 and 10% BSFP) with 3 replicates for 8 weeks. The formulae of 4 experimental diets were modified to contain 18% CP and 2,900 kcal/kg. All the treated groups differed in feed intake, egg production, egg weight and egg mass compared to those of a control group. The egg weight and production were increased (p < 0.05) in BSFP groups compared to the control. The HDL-C was increased (p < 0.05) in BSFP groups compared to the control. The level of immunoglobulin IgG and Haugh unit were increased (p < 0.05) in BSFP groups compared to the control. These result suggested that the addition of BSFP to the layer diet could improve the egg quality and the laying performance.