논문 상세보기
pp.35-41
A method for rapid micropropagation of Ophiopogon japonicus through plant regeneration from leaf, rhizome and root explants derived calli has been developed. Leaf, rhizome and root segments were cultured on Murashige and Skoog (MS) medium supplemented with plant growth regulators (2,4-D, NAA, IAA; 0~3.0 mg/ L) for callus induction. Callus production was highest at 1.0 mg/L 2,4-D where 91%. These calli were transferred to MS medium supplemented with various concentrations of 2,4-D (0, 0.5, 1.0, 3.0 mg/L) in combination with 6-benzyladenine (BA: 0, 0.5, 1.0 and 3.0 mg/L) for adventitious shoot regeneration. The addition of low concentration of 2,4-D into BA containing medium significantly increased the frequency of shoot regeneration in both leaf, rhizome and root derived calli. The highest frequency of adventitious shoot (88%) formation was on MS medium supplemented with 0.5 mg/L 2,4-D and 1.0 mg/L BA. For rooting of the shoots, half-strength MS medium supplemented with different concentrations of plant growth regulators (2,4-D, IBA, NAA) 0, 0.1, 1.0, 3.0 mg/L was tried. The optimal results was observed on half-strength MS medium supplemented with 3.0 mg/L NAA (average of 42.9 roots per explant). In vitro raised plantlets were acclimatized and transferred to soil with 100% success. This in vitro propagation protocol would be useful for conservation as well as mass propagation of this medical plant.
