The seismic isolation system reduces the seismic vibration that is transmitted from foundation to upper structure. This seismic isolation system can be classified into base isolation and mid-story isolation by the installation location. In this study, the seismic behavior of dome structure with mid-story isolation is analyzed to verify the effect of seismic isolation. Mid-story isolation is more effective than base isolation to reduce the seismic responses of roof structure. Also, this isolation would be excellent in structural characteristics and construction.