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Construction of Hyper Enhanced Baculovirus Expression Vector System

  • 언어ENG
  • URLhttps://db.koreascholar.com/Article/Detail/323127
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한국응용곤충학회 (Korean Society Of Applied Entomology)
초록

The baculovirus expression vector system (BEVS) is an effective and widely used method for the production of recombinant proteins in insect cells or larvae. However, the expression efficiency of foreign proteins using the polyhedrin promoter could not obtain the protein yields observed for native polyhedrin. To enhance the production efficiency of foreign protein in baculovirus expression system, the effects of various enhancer factor were investigated by fusion expressing them with the enhanced green fluorescent protein (EGFP). As a hyper expression factor, the optimal hyper BEVS was constructed by combining Hr5 sequence, VP39 promoter and Burst sequences. Additionally, the proteins expressed by the hyper expression system was markedly increased. This study suggests a new option for higher expression of useful foreign recombinant protein using the BEVS.

저자
  • Ji Hoon Lee(Department of Agricultural Biology, College of Agriculture, Life & Environment Science, Chungbuk National University)
  • Won Seok Gwak(Department of Agricultural Biology, College of Agriculture, Life & Environment Science, Chungbuk National University)
  • Ji In Ma(Department of Agricultural Biology, College of Agriculture, Life & Environment Science, Chungbuk National University)
  • Hyun Goo Kim(Department of Agricultural Biology, College of Agriculture, Life & Environment Science, Chungbuk National University)
  • Soo Dong Woo(Department of Agricultural Biology, College of Agriculture, Life & Environment Science, Chungbuk National University)