The technique of artificial insemination is the possible one that can surely control mating of the selected drones with the virgin queen. This technique was first time applied with A. cerana in Korean. The average amount of semen can collected from one Korean A. cerana drone was 0.09 μl and 0.1 μl of Vietnamese one, whereas the A. mellifera was more than 6 times (0.58 μl semen per A mellifera drone). Obtaining 1 μL of semen have to collect from 11.94 drones that successful semen ejection. Queens artificially inseminated with 4 μl of semen (once insemination) or 8 μl of semen twice (each insemination with 4 μl of semen) started laying egg later than naturally mated queens 5.3 and 2.5 days, respectively. The onsets of oviposition of artificially inseminated queens were 12.5 to 15.3 days.

• Yong Soo Choi(Department of Agricultural Biology, National Academy of Agricultural Science, RDA, Wanju 565-851, Republic of Korea)
• Myeong Lyeol Lee(Department of Agricultural Biology, National Academy of Agricultural Science, RDA, Wanju 565-851, Republic of Korea)
• Man Young Lee(Department of Agricultural Biology, National Academy of Agricultural Science, RDA, Wanju 565-851, Republic of Korea)
• Hye Kyung Kim(Department of Agricultural Biology, National Academy of Agricultural Science, RDA, Wanju 565-851, Republic of Korea)
• Dong Won Kim(Department of Agricultural Biology, National Academy of Agricultural Science, RDA, Wanju 565-851, Republic of Korea)
• Nguyen Ngoc Vung(Department of Agricultural Biology, National Academy of Agricultural Science, RDA, Wanju 565-851, Republic of Korea)
• Eun Jin Kang(Department of Agricultural Biology, National Academy of Agricultural Science, RDA, Wanju 565-851, Republic of Korea)