Insect growth regulators (IGRs) are insecticides that disrupt the normal development of target insects by inducing symptoms such as premature molting or supernumerary larval stages. Juvenile hormone (JH)-based IGRs are of particular interest because they simulate or interfere with the formation of juvenile hormone receptor complex. Insect larvae generally have been poorly susceptible to JH agonists (JHAs) because endogenous JH titer is already high in the larval stage. In contrast, JH antagonists (JHANs) might be more effective for control of target pests in larval stages through interference in the status quo action of JHs. In this study, we tested 2,354 chemical compounds using the yeast-two hybrid system to identify JHANs. Among them, K265674 sample was selected and its insecticidal activity was evaluated against Aedes albopictus larvae. These results may be useful for development of novel IGR insecticide.

• Seok-Hee Lee(Department of Agricultural Biotechnology, Seoul National University)
• Jae Young Choi(Research Institute for Agriculture and Life Sciences, Seoul National University)
• Jong Hoon Kim(Department of Agricultural Biotechnology, Seoul National University)
• Dong Hwan Park(Department of Agricultural Biotechnology, Seoul National University)
• Ying Fang(Department of Agricultural Biotechnology, Seoul National University)
• Min Gu Park(Department of Agricultural Biotechnology, Seoul National University)
• Ra Mi Woo(Department of Agricultural Biotechnology, Seoul National University)
• Bo Ram Lee(Department of Agricultural Biotechnology, Seoul National University)
• Woo Jin Kim(Department of Agricultural Biotechnology, Seoul National University)
• Ill Young Lee(Center for Eco-Friendly New Materials, Korea Research Institute of Chemical Technology)
• Young Kwan Ko(Center for Eco-Friendly New Materials, Korea Research Institute of Chemical Technology)
• Yeon Ho Je(Department of Agricultural Biotechnology, Seoul National University)