Rice stripe virus (RSV) is one of the serious plant pathogenic viruses for rice transmitted by small brown planthopper (SBPH), Laodalphax striatellus. RNA interference (RNAi) was recently suggested as a promising strategy for controlling insect pests, including those that serve as important vectors for plant pathogens. To disturb transmission of the RSV in SBPH using RNAi pathway, we chose nine genes highly expressed in RSV-viruliferous SBPH by transcriptome sequencing. These SBPH-derived dsRNAs were applied to the insects indirectly through xylem of rice leaves by irrigation. qPCR result demonstrated that five out of nine SBPH-derived dsRNAs successfully reduced the replication of RSV in viruliferous SBPH in dose-dependent manner, suggesting that these five dsRNAs could suppress replication of RSV and provide a new tool for RSV control strategy.

• Ying Fang(Department of Agricultural Biotechnology, Seoul National University)
• Seok-Hee Lee(Department of Agricultural Biotechnology, Seoul National University)
• Jong Hoon Kim(Department of Agricultural Biotechnology, Seoul National University)
• Dong Hwan Park(Department of Agricultural Biotechnology, Seoul National University)
• Min Gu Park(Department of Agricultural Biotechnology, Seoul National University)
• Ra Mi Woo(Department of Agricultural Biotechnology, Seoul National University)
• Bo Ram Lee(Department of Agricultural Biotechnology, Seoul National University)
• Woo Jin Kim(Department of Agricultural Biotechnology, Seoul National University)
• Jae Young Choi(Research Institute for Agriculture and Life Sciences, Seoul National University)
• Yeon Ho Je(Department of Agricultural Biotechnology, Seoul National University Research Institute for Agriculture and Life Sciences, Seoul National University)