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Increasing numbers of insecticide resistant insect is one of the main problem in current agriculture. To manage the insecticide resistance, IRAC suggest some tips for farmers : First, sequence or alteration of insecticide based on MOA. Second, choosing the effective insecticide. In this point raised following question : How to distinguish the effective or non-effective insecticide. Until now, bioassay is the right answer. However that is time-consuming, labor-intensive and costly process. So we have been steadily advancing molecular based insecticide resistance diagnosis for supporting and/or substitution of classical bioassay. It can summarized following steps : First, identification of insecticide resistance mechanism. Second, marker development. Third, development molecular diagnosis such as PASA, LAMP etc. Forth, application on field collected insects. Here we discuss some case of these study particular several major pests in highland agriculture.
