Pheromone biosynthesis in the pheromone gland is stimulated by pheromone biosynthesis activating neuropeptide (PBAN) produced in the suboesophageal ganglion. PBAN binds its receptor and transduces biological signal into the molecules for the pheromone biosynthesis. To understand pheromone biosynthesis pathway in legume pod borer, Maruca vitrata, transcriptome of the pheromone gland was analyzed. A total of 191 contigs involved in the pheromone biosynthesis were identified. Putative pheromone biosynthetic pathways for sex pheromone components in M. vitrata were proposed through transcriptomic analysis.