Dermanyssus gallinae is parasitic to chicken, and they cause many damages such as disturbing sleep reducing for body weight and egg production by blood-sucking. To develop acaricide against D. gallinae, The acaricidal activity of 40 species plant extract were examined. Cnidium officinale extracted by Me-OH showed 82.0% acaricidal activity after treated 48 hrs at 4,000 ppm. The hexane fraction showed 92.4% mortality against D. gallinae at 48 hrs at 2,000 ppm. Purification of the biologically active constituents from the hexane extraction with acaricidal activity was done using silica gel open column chromatography and HPLC. H1122 fraction gave 80.9% mortality to D. gallinae at 400 ppm after treated 48 hrs. H1122 fraction was analyzed by GC-MS and NMR.

• Seung-ju Lee(Department of plant Medicine, Chungbuk National University)
• Won Jin Kang(Department of plant Medicine, Chungbuk National University)
• Jong Ung Yoon(Biogenoci co., Ltd)
• Do Hoon Lee(Biogenoci co., Ltd)
• Hyun Kyung Kim(Department of plant Medicine, Chungbuk National University)
• Hyun-Na Koo(Department of plant Medicine, Chungbuk National University)
• Gil-Hah Kim(Department of plant Medicine, Chungbuk National University)