This study was conducted to determine the effect of rainfall after cutting on nutritive value of Italian ryegrass (Lolium multiflorum) hay. Fresh Italian ryegrass was treated with 3 days natural rainfall (56.5, 14.0 and 1.6 mm) after cutting without tedding. Rainfall effect on Italian ryegrass quality was evaluated based on Neutral detergent fiber (NDF) and acid detergent fiber (ADF), crude protein (CP), crude fat (CF), crude ash (CA) and non-fiber carbohydrate (NFC). The quality of Italian ryegrass was decreased with extending raining days. The content of neutral detergent fiber (NDF) was increased from 0.4 to 4.7% by 1 day advancement in raining. CF content was decreased from 8.1 to 32.8%. CP and CA contents did not change, but NFC content was decreased from 0.5 to 8.4%. The quality damage by the number of raining days after cutting Italian ryegrass was the lowest on the first day from exposure to rain.