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pp.946-951
In this study, Ilex serrata Thunb was extracted with 70% methanol and concentrated under reduced pressure to measure its total polyphenol contents; furthermore, we examined the effect of electron donating ability of the various extracts. The extract was fractionated using different solvents such as methylene chloride, ethyl acetate, and methanol to investigate total polyphenol contents and electron donating abilities. The total polyphenol contents were 1,058.59±85.85 μg GAE/mL in 70% methanol, 297.29±29.43 μg GAE/mL in methylene chloride, 313.28±9.22 μg GAE/mL in ethyl acetate, and 837.82±31.16 μg GAE/mL in methanol. The total polyphenol contents showed significant differences (p<0.05) between the solvents. The electron donating abilities were 72.93±0.29% for 70% methanol extract, 14.02±2.01% for methylene chloride, 24.05±1.75% for ethyl acetate, and 84.08±1.95% for methanol. The electron donating abilities were significantly different (p<0.05) between the solvents. The two portions of Ilex serrata Thunb extracts obtained from methanol showed significantly relevant results in the total polyphenol contents and electron donating ability, which was higher than the original extract
